產(chǎn)品說(shuō)明 ：
產(chǎn)品貨號(hào)Reference: CAN-SHBG-4010
檢測(cè)方法Kit Type: Sandwich ELISA
規(guī)格Kit Size: 96-well break-apart microplate
靈敏度Sensitivity: 0.1 nmol/L
檢測(cè)樣本Sample Type: Human serum / 10 μL
檢測(cè)范圍Calibrator Range: 3.3–295 nmol/L
檢測(cè)時(shí)間Total Assay Time: 60 minutes
背景介紹：
性激素結(jié)合球蛋白(SHBG)是由373個(gè)氨基酸殘基和3個(gè)糖鏈組成的糖蛋白。SHBG被命名為睪酮-雌二醇結(jié)合球蛋白(TeBG)、性類固醇結(jié)合蛋白(SBP)和性類固醇結(jié)合球蛋白(SSBG)。其主要特性之一是其對(duì)甾體的高親和力，尤其是C18、C19和17個(gè)α-羥基。甾體與SHBG的結(jié)合具有溫度和pH依賴性。對(duì)SHBG有高親和力的三種類固醇是雙氫睪酮、睪酮和雌二醇。極少量的類固醇在生物液中是游離的；大多數(shù)是與SHBG和白蛋白結(jié)合的。當(dāng)SHBG濃度變化時(shí)，無(wú)論是白蛋白結(jié)合的激素還是游離部分，都發(fā)生了顯著的變化。
Sex hormone binding globulin (SHBG) is a glycoprotein composed of 373 amino acid residues and three carbohydrate side chains. SHBG has been known by many other names including Testosterone-estradiol Binding Globulin (TeBG), Sex steroid Binding Protein (sBP) and Sex Steroid Binding Globulin (SSBG). One of the main properties of SHBG is its high affinity for steroids, especially the C18, C19 and 17α-hydroxyl groups. The binding of steroids to SHBG is temperature and pH dependent. The three steroids that have a high avidity for SHBG are Dihydrotestosterone, Testosterone and Estradiol. Very small amounts of these steroids are free in biological fluid; the majority are bound to SHBG and albumin. These two fractions, that is, free and bound exist in a state of dynamic equilibrium. When the level of SHBG concentration changes, a remarkable change occurs in both albumin-bound hormone and also in the free fraction.
