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產(chǎn)品貨號(hào)Reference:              CAN-E-7030
檢測(cè)方法Kit Type:                 Competitive LIA
規(guī)格Kit Size:                         96-well break-apart microplate
靈敏度Sensitivity:                 0.03 ng/mL
檢測(cè)樣本Sample Type:         Human saliva / 50 μL
檢測(cè)范圍Calibrator Range:  0.03–30 ng/mL
檢測(cè)時(shí)間Total Assay Time:  95 minutes
背景介紹：
在妊娠期間，雌三醇的測(cè)定用于監(jiān)測(cè)胎兒的健康狀況。因此，在懷孕期間，雌三醇水平可以檢測(cè)到胎兒發(fā)育的異常。隨著懷孕時(shí)間的推移，雌三醇的濃度也會(huì)增加。唾液中未結(jié)合雌三醇的含量反映了血清中未結(jié)合雌三醇的濃度，因此唾液中未結(jié)合雌三醇的測(cè)定對(duì)跟蹤妊娠的進(jìn)展具有重要的臨床意義。唾液中的未結(jié)合雌三醇水平反映了?的生物活性部位。
唾液的收集與尿液和血清相比有許多優(yōu)點(diǎn)，因?yàn)樗且环N非侵入性的程序，可以很容易地在家里收集和冷凍儲(chǔ)存。
由于唾液中未結(jié)合雌三醇的濃度較低，我們建立了一種基于化學(xué)發(fā)光免疫分析(LIA)的檢測(cè)系統(tǒng)。該方法靈敏度高(0.03 ng/mL)，準(zhǔn)確、準(zhǔn)確，在搖床上的RT°孵育時(shí)間為75分鐘。
During pregnancy the determination of estriol is used for monitoring fetal well being. Therefore, during pregnancy the estriol level can detect abnormalities in the development of the fetus. As pregnancy progresses in time the concentration of Estriol also increases. The unconjugated estriol saliva determination is of great clinical importance to follow the progress of pregnancy since the level of salivary unconjugated estriol reflects the unconjugated estriol concentration in serum, which re? ects the biologically active fraction.
The collection of saliva has advantages compared to urine and serum since it is a non-invasive procedure which can be easily collected at home and stored frozen.
Due to the low concentration of unconjugated estriol in saliva we have developed an assay system using a chemiluminescent immunoassay (LIA). The assay is very sensitive (0.03 ng/mL), precise and accurate along with a short incubation time of 75 minutes at RT° on a shaker.

注：本產(chǎn)品僅用于科研用途，不能直接用于人體或臨床診斷使用。